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Archaeal communities associated with shallow to deep subseafloor sediments of the New Caledonia Basin ArchiMer
Roussel, Erwan; Sauvadet, Anne-laure; Chaduteau, Carine; Fouquet, Yves; Charlou, Jean-luc; Prieur, Daniel; Cambon, Marie-anne.
P>The distribution of the archaeal communities in deep subseafloor sediments [0-36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average similar to 3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0-1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured...
Tipo: Text Palavras-chave: Marine subsurface sediments; Sea floor biosphere; Gradient gel electrophoresis; Microbial communities; Molecular diversity; Anaerobic oxidation; Sequence alignment; Phylogenetic trees; Extracellular DNA; Methane hydrate.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6801.pdf
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Biogeochemistry and community composition of iron- and sulfur-precipitating microbial mats at the Chefren mud volcano (Nile Deep Sea fan, Eastern Mediterranean) ArchiMer
Omoregie, E; Mastalerz, V; De Lange, G; Straub, K; Kappler, A; Roy, H; Stadnitskaia, A; Foucher, Jean-paul; Boetius, A.
In this study we determined the composition and biogeochemistry of novel, brightly colored, white and orange microbial mats at the surface of a brine seep at the outer rim of the Chefren mud volcano. These mats were interspersed with one another, but their underlying sediment biogeochemistries differed considerably. Microscopy revealed that the white mats were granules composed of elemental S filaments, similar to those produced by the sulfide-oxidizing epsilonproteobacterium "Candidatus Arcobacter sulfidicus." Fluorescence in situ hybridization indicated that microorganisms targeted by a "Ca. Arcobacter sulfidicus"-specific oligonucleotide probe constituted up to 24% of the total the cells within these mats. Several 16S rRNA gene sequences from organisms...
Tipo: Text Palavras-chave: Gulf of mexico; Sulfate reducing bacteria; Egyptian continental margin; Northeast pacific ocean; Ribosomal rna sequences; In situ hybridization; Anaerobic oxidation; Marine sediments; Gas hydrate; Oxiding bacteria.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6201.pdf
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Methane in shallow subsurface sediments at the landward limit of the gas hydrate stability zone offshore Western Svalbard ArchiMer
Graves, Carolyn A.; James, Rachael H.; Sapart, Celia Julia; Stott, Andrew W.; Wright, Ian C.; Berndt, Christian; Westbrook, Graham; Connelly, Douglas P..
Offshore western Svalbard plumes of gas bubbles rise from the seafloor at the landward limit of the gas hydrate stability zone (LLGHSZ; ∼400 m water depth). It is hypothesized that this methane may, in part, come from dissociation of gas hydrate in the underlying sediments in response to recent warming of ocean bottom waters. To evaluate the potential role of gas hydrate in the supply of methane to the shallow subsurface sediments, and the role of anaerobic oxidation in regulating methane fluxes across the sediment-seawater interface, we have characterised the chemical and isotopic compositions of the gases and sediment pore waters. The molecular and isotopic signatures of gas in the bubble plumes (C1/C2+ = 1 × 104; δ13C-CH4 = -55 to -51 ‰; δD-CH4 = -187...
Tipo: Text Palavras-chave: Methane; Seafloor sediments; Gas hydrate; Offshore Svalbard; Seabed fluxes; Anaerobic oxidation.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00358/46914/46819.pdf
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Structure and Drivers of Cold Seep Ecosystems ArchiMer
Foucher, Jean-paul; Westbrook, Graham K.; Boetius, Antje; Ceramicola, Silvia; Dupre, Stephanie; Mascle, Jean; Mienert, Jurgen; Pfannkuche, Olaf; Pierre, Catherine; Praeg, Daniel.
Submarine hydrocarbon seeps are geologically driven "hotspots" of increased biological activity on the seabed. As part of the HERMES project, several sites of natural hydrocarbon seepage in the European seas were investigated in detail, including mud volcanoes and pockmarks, in study areas extending from the Nordic margin, to the Gulf of Cadiz, to the Mediterranean and Black seas. High-resolution seabed maps and the main properties of key seep sites are presented here. Individual seeps show ecosystem zonation related to the strength of the methane flux and distinct biogeochemical processes in surface sediments. A feature common to many seeps is the formation of authigenic carbonate constructions. These constructions exhibit various morphologies ranging...
Tipo: Text Palavras-chave: Mosby mud volcano; Deep sea fan; Eastern mediterranean sea; Black sea; Anaerobic oxidation; Hydrocarbon seeps; Carbonate crusts; Sidescan sonar; Hydrate ridge; Gas chimneys.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6387.pdf
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